The Ashton Estate | OHL Limited

Dr Charles Daniel Lane

CDL Sector, Ashton Estate

Charles’ University of Oxford doctoral thesis entitled “The Microinjection of RNA into Eggs and Oocytes of Xenopus Laevis” yielded interesting results .

The discovery that living cells programmed with exogenous messenger RNA can make and correctly modify foreign proteins is, for three separate reasons, arguably one of the most important scientific advances of the last 50 years. Thus:

  1. The foreign mRNA can reveal controlled mechanisms within the living cell.
  2. Foreign proteins made within a living cell provide a “living test tube” for the discovery and study of molecules, including many pharmaceuticals – such as those that interact with receptor proteins embedded in cell membranes.
  3. Messenger RNA Therapeutics may revolutionize many conventional medical treatments – from anti-viral vaccines to heart medicines to cancer vaccines.  However, to date no mRNA Therapeutic has yet reached the market place 

Wikipedia Page: See https://en.wikipedia.org/wiki/Charles_Daniel_Lane ;and we have listed some of Dr Lane’s scientific papers here:

  1. 1971 Rabbit Haemoglobin Synthesis in Frog Cells
  2. Translational
  3. The use of frog eggs and oocytes for the study of messenger RNA and its translation
  4. 9S RNA from reticulocytes and its assay if frog oocytes
  5. Traduction du RNA 9S de reticulocytes de lapin en chaines de globine dans les oeufs et les oocytes de xenope
  6. The translation of reticulocyte 9s RNA in frog oocytes gives ris to alpha and beta globin chains
  7. Rabbit haemoglobin in frog cells II Further characterixation of the products of translation of reticocyte 9 s RNA
  8. Calf Crystallin Synthesis in Frog Cells The Translation of Lenscells 14SRNA in Oocytes
  9. Some characteristics of Gene Expression as revealed by a living assay system
  10. Translation of Eukaryotic Messenger RNA in various heterologous systems
  11. Duck haemoglobin synthesis in frog cells the translation and assay of reticulocyte 9-S RNA in occutes of Xenopus
  12. The translation of Messenger RNA for Mouse Immunoglobulin Light Chais in Living Frog Oocytes
  13. The use of the xenopus oocyte to prove the existence of messenger sequences in high molecular weight rna
  14. The injection of rna into living cells the use of frog oocytes for assay of mrna and the tudy of the control of gene expression
  15. Control of translation of globin mRNA in embryonic cells
  16. Insect protein synthesis in Frog Cells The translation of honey bee promelittin messenger rna in xenopus oocytes
  17. Electrophoretic separation of the major species of slime mold messenger RNA
  18. Fractionation of mRNAs from Dictyostelium Discoideum
  19. Translation of Xenopus live messenger RNA in Xenopus oocytes vitellogenin synthesis and coversion to yolk platelet proteins
  20. CDL Scientific American article with advert
  21. Subcellular compartmentation of albumin and globin made in oocytes under the direction of injected messenger RNA.
  22. Requirement for 7-methylguanosine in translation of globin mRNA in vivo
  23. Synthesis and processing of milk proteins 12th FEBS meeting
  24. Sequestration and Turnover of Guinea-pig Milk Proteins and Chicken Ovalbumin in Xenopus Oocytes
  25. The Xenopus oocyte as a surrogate secretory system
  26. The Influence of Topology and Glycosylation on the Fate of Heterologous Secretory Proteins Made in Xenopus Oocytes
  27. The Sequestration, Processing and Retention of Honey-Bee Promelittin Made in Amphibian Oocytes
  28. Fate of secretory proteins trapped in oocytes of Xenopus laevis by disruption of the cytoskeleton or by imbalanced subunit synthesis
  29. The secretion of proteins in vitro from Xenopus oocytes and their accessory cells a biochemical and morphological study
  30. Synthesis and Insertion, both in vivo and in vitro, of Rat-Liver Cytochrome P-450 and Epoxide Hydratase into Xenopus Zaevis Membranes
  31. Non-parallel kinetics and the role of tissue-specific factors in the secretion of chicken ovalbumin and lysozyme from Xenopus oocytes
  32. The Fate of Foreign Proteins Introduced into Xenopus Oocytes
  33. Actin synthesis during the early development of Xenopus laevis
  34. The Fate of Xenopus and Locust Vitellogenins Made in Xenopus Oocytes An Export-Import Processing Model
  35. Signal sequences, secondary modification and the turnover of miscompartmentalized secretory proteins in Xenopus oocytes.
  36. The fate of genes, messengers and proteins introduced intoXenopus oocytes
  37. The absence from the oocyte secretory system of a protein kinase capable of phophorylating sequestered caseins